Welcome to BWH Transgenic Core

Gene Targeting Application Form Services & Fees Technical Contacts Address
　
　 The Brigham & Women's Hospital  Transgenic Core Facility provides services for generating transgenic and knockout mice.

The services include the following:

1.Microinjection of DNA constructs (including BACs) into pronuclei for generating transgenic mice (FVB or C57 BL/6 strain background)
2.Microinjection of embryonic stem (ES) cells into blastocysts for generating knockout mice
3.ES cell transfection for generating ES cell clones with the targeted combination event

    Established in 1992, the BWH Core Facility has been providing gene targeting and microinjection services using state-of-the-art facilities and equipment. Dr. Lina Du, formerly a plastic surgeon in Beijing and an expert in microvascular surgery, performs both blastocyst and pronuclear injections. She has over 15 years of experience in embryo manipulation and the generation transgenic mice. The Core Facility has all of the equipment necessary for generation of transgenic mice including a Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation ad transfer, a sutter needle puller and a de Fonbrune microforge. The facility maintains mice necessary for egg donors, egg recipients, and vasectomized males. This is a non-profit facility and charges are based upon anticipated mouse costs, maintenance of mice and equipment, and purchase of necessary surgical supplies and chemicals.

    The ES cell culture service has been offered for 6 years. Xiaomeng Tang and Matthew Finn carry out electroporation of targeting vectors into ES cells and provides investigators with DNA to identify ES cells carrying the desired recombination events. Then they expand the appropriate ES Cells for microinjection into blastocysts by the BWH Transgenic Core.